pgl3 rare responsive promoter Search Results


pgl3 traf6 promoter luciferase  (Addgene inc)
93
Addgene inc pgl3 traf6 promoter luciferase
Pgl3 Traf6 Promoter Luciferase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pm25607648-146-31-40?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pgl3 traf6 promoter luciferase - by Bioz Stars, 2026-08
93/100 stars
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luciferase reporter gene vector pgl3-basic  (Promega)
90
Promega luciferase reporter gene vector pgl3-basic
Luciferase Reporter Gene Vector Pgl3 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/us08217007-1022-11-16?v=Promega
Average 90 stars, based on 1 article reviews
luciferase reporter gene vector pgl3-basic - by Bioz Stars, 2026-08
90/100 stars
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pgl3 enhancer foxo3a promoter vector  (Addgene inc)
93
Addgene inc pgl3 enhancer foxo3a promoter vector
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Pgl3 Enhancer Foxo3a Promoter Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pmc05427268-725-7-19?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pgl3 enhancer foxo3a promoter vector - by Bioz Stars, 2026-08
93/100 stars
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3xly6e/pzluc-tk luciferase reporter plasmid  (Promega)
90
Promega 3xly6e/pzluc-tk luciferase reporter plasmid
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
3xly6e/Pzluc Tk Luciferase Reporter Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pmc03702291-170-3-4?v=Promega
Average 90 stars, based on 1 article reviews
3xly6e/pzluc-tk luciferase reporter plasmid - by Bioz Stars, 2026-08
90/100 stars
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pgl 3 plasmids  (Addgene inc)
95
Addgene inc pgl 3 plasmids
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Pgl 3 Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pm36007676-68-4-14?v=Addgene+inc
Average 95 stars, based on 1 article reviews
pgl 3 plasmids - by Bioz Stars, 2026-08
95/100 stars
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reporter plasmid of full-length promoter construct (wild-type or mutant) of pgl-3-ftmt  (Shanghai GenePharma)
90
Shanghai GenePharma reporter plasmid of full-length promoter construct (wild-type or mutant) of pgl-3-ftmt
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Reporter Plasmid Of Full Length Promoter Construct (Wild Type Or Mutant) Of Pgl 3 Ftmt, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pmc05762514-101-10-14?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
reporter plasmid of full-length promoter construct (wild-type or mutant) of pgl-3-ftmt - by Bioz Stars, 2026-08
90/100 stars
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pgl-3-tgfb1  (Shanghai GenePharma)
90
Shanghai GenePharma pgl-3-tgfb1
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Pgl 3 Tgfb1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pmc05349884-109-12-16?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
pgl-3-tgfb1 - by Bioz Stars, 2026-08
90/100 stars
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luciferase gene sequence  (Promega)
90
Promega luciferase gene sequence
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Luciferase Gene Sequence, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/10__1074_slash_jbc__m413528200-63-21-23?v=Promega
Average 90 stars, based on 1 article reviews
luciferase gene sequence - by Bioz Stars, 2026-08
90/100 stars
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pgl-3-basic  (Promega)
90
Promega pgl-3-basic
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Pgl 3 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/bio_rxiv__2020__04__24__060236-257-11-12?v=Promega
Average 90 stars, based on 1 article reviews
pgl-3-basic - by Bioz Stars, 2026-08
90/100 stars
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pgl-3.0-basic  (Promega)
90
Promega pgl-3.0-basic
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Pgl 3.0 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pm32106376-94-18-19?v=Promega
Average 90 stars, based on 1 article reviews
pgl-3.0-basic - by Bioz Stars, 2026-08
90/100 stars
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pgl 3-control  (Promega)
90
Promega pgl 3-control
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Pgl 3 Control, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/10__1042_slash_bsr20190108-43-14-16?v=Promega
Average 90 stars, based on 1 article reviews
pgl 3-control - by Bioz Stars, 2026-08
90/100 stars
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pgl-3 plasmid  (BioChain Institute)
90
BioChain Institute pgl-3 plasmid
<t>FoxO3a</t> regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with <t>pGL3-enhancer</t> FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.
Pgl 3 Plasmid, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+rare+responsive+promoter/pmc03024581-365-54-63?v=BioChain+Institute
Average 90 stars, based on 1 article reviews
pgl-3 plasmid - by Bioz Stars, 2026-08
90/100 stars
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FoxO3a regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with pGL3-enhancer FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.

Journal: The Journal of Biological Chemistry

Article Title: AMP-activated protein kinase α1 promotes atherogenesis by increasing monocyte-to-macrophage differentiation

doi: 10.1074/jbc.M117.779447

Figure Lengend Snippet: FoxO3a regulates AMPKα1 dependent autophagy induction. A, protein expression of Foxo3a in bone marrow-derived macrophages in WT or AMPKα1−/− mice after 5 days of M-CSF treatment. #, p < 0.01 versus WT. Data are representative of five independent experiments. B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.05 versus WT. Data are representative of three independent experiments. C, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h. Protein expression of Foxo3a was detected by Western blotting. #, p < 0.01 versus control siRNA. Data are representative of three independent experiments. D, THP1 cells were transfected with AMPKα1 or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of FoxO3a. #, p < 0.01 versus control (n = 6). E, HEK293T cells were co-transfected with pGL3-enhancer FoxO3a promoter vector in combination with pCMV-AMPKα1 plasmid or control plasmid. Renilla was used as an internal control. After 24 h of transfection, the cells were analyzed for firefly luciferase and Renilla luciferase activity using the Dual-Luciferase reporter assay. Data are expressed as the -fold increase over mock-transfected cells. #, p < 0.01 versus control (n = 6). F, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of LC3. #, p < 0.01 versus control siRNA (n = 6). G, THP1 cells were transfected with FoxO3a siRNA for 48 h and then treated with or without 5 ng/ml bafilomycin A1 for 6 h. Protein expression of LC3 was detected by Western blotting. #, p < 0.01 versus control siRNA; *, p < 0.01 versus control siRNA with bafilomycin A1 treatment. Data are representative of three independent experiments. H, THP1 cells were transfected with FoxO3a siRNA or control siRNA for 48 h, and ULK1 protein expressions were detected by Western blotting. p < 0.01 versus control siRNA. Data are representative of three independent experiments. I, THP1 cells were transfected with FoxO3a or control siRNA for 48 h, and real-time RT-PCR was performed to quantify mRNA levels of ULK1. #, p < 0.01 versus control (n = 6). Error bars, S.E.

Article Snippet: Luciferase assay HEK293T cells were co-transfected with pGL3-enhancer FoxO3a promoter vector (FHRE-Luc was a gift from Dr. Michael Greenberg (Addgene plasmid 1789) in combination with the pCMV-AMPKα1 plasmid (Origene, catalog no. RC218572) or empty plasmid.

Techniques: Expressing, Derivative Assay, Isolation, Injection, Western Blot, Transfection, Control, Quantitative RT-PCR, Plasmid Preparation, Luciferase, Activity Assay, Reporter Assay

Autophagy activation by rapamycin promotes monocyte differentiation in AMPKα1-deleted mice through up-regulation of FoxO3 activity. A, HEK293T cells were transfected with pGL3-enhancer FoxO3a promoter vector for 24 h. The cells were then treated with 500 nm rapamycin for 4 h. Firefly luciferase and Renilla luciferase activity were detected by using the Dual-Luciferase reporter assay. #, p < 0.01 versus control (n = 3). B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. The cells were treated with or without 500 nm rapamycin for 24 h. Protein expressions of Foxo3a and LC3 were detected by Western blotting. #, p < 0.05 versus WT with vehicle treatment. *, p < 0.05 versus AMPKα1−/− with vehicle treatment. Data are representative of three independent experiments. C, bone marrow-derived macrophages from WT or AMPKα1−/− mice were treated with vehicle or 250 nm rapamycin for 6 h. #, p < 0.05 versus WT with vehicle treatment; *, p < 0.05 versus AMPKα1−/− with vehicle treatment. Data are representative of three independent experiments. D, detection of Ly6Clo/F4/80hi and Ly6Chi/F4/80lo cells by flow cytometry in peritoneal cells after 3 days of thioglycollate injection in ApoE−/− and ApoE−/−/AMPKα1−/− mice, which were subcutaneously injected with vehicle or 6 mg/kg/day rapamycin for 10 days; #, p < 0.05 versus Ly6Chi/F4/80lo ApoE−/− cells; *, p < 0.01 versus Ly6Chi/F4/80lo ApoE−/−/AMPKα1−/− cells; †, p < 0.05 versus F4/80+ ApoE−/− cells; ‡, p < 0.01 versus F4/80+ ApoE−/−/AMPKα1−/− cells (n = 3–5). E, macrophage (F4/80+) numbers in peritoneal cavity 3 days after thioglycollate injection in ApoE−/− and ApoE−/−/AMPKα1−/− mice, which were subcutaneously injected with vehicle or 6 mg/kg/day rapamycin for 10 days. *, p < 0.01 versus vehicle treatment in ApoE−/− mice (n = 3–5). Error bars, S.E.

Journal: The Journal of Biological Chemistry

Article Title: AMP-activated protein kinase α1 promotes atherogenesis by increasing monocyte-to-macrophage differentiation

doi: 10.1074/jbc.M117.779447

Figure Lengend Snippet: Autophagy activation by rapamycin promotes monocyte differentiation in AMPKα1-deleted mice through up-regulation of FoxO3 activity. A, HEK293T cells were transfected with pGL3-enhancer FoxO3a promoter vector for 24 h. The cells were then treated with 500 nm rapamycin for 4 h. Firefly luciferase and Renilla luciferase activity were detected by using the Dual-Luciferase reporter assay. #, p < 0.01 versus control (n = 3). B, peritoneal macrophages were isolated 3 days after thioglycollate injection in WT or AMPKα1−/− mice. The cells were treated with or without 500 nm rapamycin for 24 h. Protein expressions of Foxo3a and LC3 were detected by Western blotting. #, p < 0.05 versus WT with vehicle treatment. *, p < 0.05 versus AMPKα1−/− with vehicle treatment. Data are representative of three independent experiments. C, bone marrow-derived macrophages from WT or AMPKα1−/− mice were treated with vehicle or 250 nm rapamycin for 6 h. #, p < 0.05 versus WT with vehicle treatment; *, p < 0.05 versus AMPKα1−/− with vehicle treatment. Data are representative of three independent experiments. D, detection of Ly6Clo/F4/80hi and Ly6Chi/F4/80lo cells by flow cytometry in peritoneal cells after 3 days of thioglycollate injection in ApoE−/− and ApoE−/−/AMPKα1−/− mice, which were subcutaneously injected with vehicle or 6 mg/kg/day rapamycin for 10 days; #, p < 0.05 versus Ly6Chi/F4/80lo ApoE−/− cells; *, p < 0.01 versus Ly6Chi/F4/80lo ApoE−/−/AMPKα1−/− cells; †, p < 0.05 versus F4/80+ ApoE−/− cells; ‡, p < 0.01 versus F4/80+ ApoE−/−/AMPKα1−/− cells (n = 3–5). E, macrophage (F4/80+) numbers in peritoneal cavity 3 days after thioglycollate injection in ApoE−/− and ApoE−/−/AMPKα1−/− mice, which were subcutaneously injected with vehicle or 6 mg/kg/day rapamycin for 10 days. *, p < 0.01 versus vehicle treatment in ApoE−/− mice (n = 3–5). Error bars, S.E.

Article Snippet: Luciferase assay HEK293T cells were co-transfected with pGL3-enhancer FoxO3a promoter vector (FHRE-Luc was a gift from Dr. Michael Greenberg (Addgene plasmid 1789) in combination with the pCMV-AMPKα1 plasmid (Origene, catalog no. RC218572) or empty plasmid.

Techniques: Activation Assay, Activity Assay, Transfection, Plasmid Preparation, Luciferase, Reporter Assay, Control, Isolation, Injection, Western Blot, Derivative Assay, Flow Cytometry